Jacalin-related lectins (JRLs) are a new subfamily of plant lectins that has been recognized in recent years, and they are widely distributed among plant species ( Bourne et al., 2004). Lectins play an important role as storage proteins ( Barbieri et al., 2004) and participate in biological nitrogen fixation, the promotion of cell division ( Jung et al., 2007), endogenous regulation ( Xin et al., 2009), and abiotic stress response ( Zhang et al., 2000 Ray et al., 2012).
BLACK MOSO BAMBOO GROWTH RATE SERIES
They contain at least one non-catalytic structural domain capable of reversibly binding sugars, and they can trigger a series of downstream biochemical cascades by binding to specific carbohydrates ( Peumans and Van Damme, 1995 Sharon, 2007). Plant lectins are a structurally complex class of sugar-binding proteins that can be divided into several families ( Damme et al., 2008). These findings provide an important reference for understanding the functions of specific moso bamboo JRL genes and for the effective selection of stress-related genes.
Three dimensional structure prediction and yeast two-hybrid assays were used to verify that PeJRL13 exists as a self-interacting homodimer in vivo. Subcellular localization experiments revealed that PeJRL04 and PeJRL13 were localized to the cell membrane, nucleus, and cytoplasm. qRT-PCR expression analysis showed that JRL genes in the same subgroup were significantly downregulated in response to salicylic acid (SA), abscisic acid (ABA), and methyl jasmonate (MeJA) treatments and significantly upregulated under low temperature, drought, and salt stress they also exhibited tissue-specific expression patterns. Sixty-four percent of the PeJRL genes are present as segmental and tandem duplicates. Genome collinearity analyses showed that the JRL genes of moso bamboo are more closely related to those of Brachypodium distachyon than to those of Oryza sativa and Zea mays.
Numerous stress-responsive and hormone-regulated cis-elements were detected in the upstream promoter regions of the JRLs. Phylogenetic analysis showed that the moso bamboo JRLs were clustered into four JRL subgroups: I, II, V, and VII.
Here, we identified 25 JRL genes in moso bamboo, and these genes are unequally distributed among 10 genome scaffolds. Although moso bamboo ( Phyllostachys edulis) is an economically and industrially important bamboo worldwide, there has been no systematic identification of JRLs in this species. Jacalin-related lectins (JRLs) are a new subfamily of plant lectins that has recently been recognized and plays an important role in plant growth, development, and abiotic stress response.
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